Hypoxia-Induced Overexpression Of Alpha Lpha5 Nicotinic Acetylcholine Receptor Of Human Lung Cancer Cell Lines
2014 IEEE WORKSHOP ON ELECTRONICS, COMPUTER AND APPLICATIONS(2014)
摘要
Previous studies have indicated that alphaS nicotinic acetylcholine receptor (alpha 5-nAChR) is highly associated with lung cancer risk and nicotine dependence. However, the mechanisms through which alpha 5-nAChRs may influence lung carcinogenesis are far from clear. Studies also showed that the ability of nicotine to drive the acquisition of a malignant phenotype characterized by an increased risk of metastasis is mostly similar to that of hypoxia. However, little is known concerning the effects of hypoxia on the expression of HIF-1 alpha and alpha 5-nAChR in human lung cancer cells. In the present study, RT-PCR and western blotting were conducted to assay the mRNA and protein levels of HIF-1 alpha and alpha 5-nAChR under normoxia and hypoxia induced by exposure to 0.5% O-2 for 24 h and 48 h in lung cancer A549 cells. Results showed that hypoxia induced the up regulation of HIF-1 alpha, in a time-dependent manner, which was also observed at the alpha 5-nAChR mRNA and protein levels (P<0.05). A positive correlation was found between HIF-1 alpha, and alpha 5-nAChR expression in both H1975 and H1299 cells. We suggest that hypoxia induced the overexpression of as-nAChR, the mechanism of which may be related to the upregulation of HIF-1 alpha in lung cancer cells. The relationship between HIF-1 alpha and alpha 5-nAChR expression as a possible treatment for lung cancer cells should be assessed in clinical trials.
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关键词
Hypoxia, HIF-1 alpha, alpha 5-nAChR, Lung cancer cells
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