A [2fe-2s] Protein Encoded By An Open Reading Frame Upstream Of The Escherichia Coli Bacterioferritin Gene

An open reading frame located upstream of the bacterioferritin gene in Escherichia call encodes a hypothetical 64-residue protein [Andrews, S. C., Harrison, P. C., & Guest, J. R. (1989) J. Bacterial. 171, 3940-3947)]. The spacing of the four cysteine residues in this hypothetical protein is identical to that in a region of NIFU, a [2Fe-2S] protein found in nitrogen-fixing bacteria [Fu, W., Jack, R. F., Morgan, T, V., Dean, D. R., & Johnson, M. K. (1994) Biochemistry 33, 13455-13463)]. The NIFU-like E, call gene was cloned and overexpressed with a C-terminal ''His tag'' in E. call using the T7 RNA polymerase/ promoter system, and the protein was purified by metal-chelate affinity chromatography. UV-vis absorption and EPR spectra together with iron and amino acid analyses conclusively established that this NIFU-like E, call protein contains one [2Fe-2S] cluster which can exist in at least two oxidation levels: +2 for the as-purified protein, and +1 for dithionite-reduced protein, Size-exclusion chromatography established that this His-tagged [2Fe-2S] protein is monomeric in solution. Affinity chromatography demonstrated specific complex formation between bacterioferritin (Bfr) and this NIFU-like [2Fe-2S] protein, which is dubbed Bfd. An open reading frame encoding a homologous Bfd is located near a Bfr gene in at least one other bacterium. Bfd may, therefore, constitute a general redox and/or regulatory component participating in the iron storage or mobilization functions of Bfr.